| abstract |
The present invention provides compositions and methods for reprogramming somatic cells using a purified RNA preparation comprising single-stranded mRNA encoding an iPS cell inducer. The purified RNA preparation is preferably substantially free of RNA contaminant molecules, which i) can activate an immune response in somatic cells, ii) reduce the expression of single-stranded mRNA in somatic cells, and/or iii) It will be able to activate RNA sensors in somatic cells. In some embodiments, the purified RNA preparation is substantially free of partial mRNA, double stranded RNA, uncapped RNA molecules, and/or single stranded run-on mRNA. |