| abstract |
The present invention provides a method for producing exosomes comprising culturing animal-derived cells in a medium containing TNF-α and interferon-γ. According to the present invention, it is possible to not only improve the productivity of exosomes isolated from cell culture fluid, but also exhibit an excellent effect of enhancing physiological activity such as increasing elastin synthesis of exosomes. Therefore, the method for producing exosomes of the present invention can economically and efficiently produce exosomes with enhanced physiological activity that are commercially and/or clinically useful in high yield, and in particular, have much superior physiological activity compared to conventional methods. It has the advantage of being able to mass-produce exosomes. |