| abstract |
The present invention has a 1,3-BDO pathway with at least one foreign nucleic acid encoding a 1,3-BDO pathway enzyme expressed in an amount sufficient to produce 1,3-butanediol (1,3-BDO). Non-naturally occurring microbial organisms, including microbial organisms. The pathway is 2-amino-4-ketopentanoate (AKP) thiolase, AKP dehydrogenase, 2-amino-4-hydroxypentanoate aminotransferase, 2-amino-4-hydroxypentanoate oxidoreductase (deamination), 2-oxo-4-hydroxypentanoate decarboxylase, 3-hydroxybutyraldehyde reductase (reductase), AKP aminotransferase, AKP oxidoreductase (deamination), 2,4-dioxopentanoate decarboxylase, 3-oxobutyraldehyde reductase (ketone reduction), 3-oxobutyr Aldehyde reductase (aldehyde reduction), 4-hydroxy-2-butanone reductase, AKP decarboxylase, 4-aminobutan-2-one aminotransferase, 4-aminobutan-2-one oxidoreductase ( Deamination), 4-aminobutan-2-one ammonia-lyase, butenone hydratase, AKP ammonia-lyase, acetylacrylate decarboxylase, acetoacetyl-CoA reductase (CoA -Dependence, aldehyde formation), acetoacetyl-CoA reductase (CoA-dependence, alcohol formation), acetoacetyl-CoA reductase (ketone reduction), 3-hydroxybutyryl-CoA reductase (aldehyde formation), 3- And enzymes selected from the group consisting of hydroxybutyryl-CoA reductase (alcohol formation), 4-hydroxybutyryl-CoA dehydratase, and crotonase. The method of production of 1,3-BDO involves culturing such a non-naturally occurring microbial organism under conditions for a period sufficient to produce 1,3-BDO. |