http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102050870-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P5-026 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-69 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-46 |
| filingDate | 2018-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-102050870-B1 |
| titleOfInvention | Modulation of plasmid copy number in antibiotics-free plasmid maintenance system |
| abstract | The present invention provides a synthetic 5 ′ untranslated region (UTR), a gene expression cassette comprising a promoter and a regulatory gene, an origin of replication and a recombinant vector comprising the gene expression cassette, and improved segregational instability into which the recombinant vector is introduced. The present invention relates to a method for preparing a recombinant microorganism, a recombinant microorganism having improved separation anxiety by introducing the recombinant vector, and a method for quantitatively controlling the number of plasmid copies in a recombinant microorganism. InfA , a gene encoding a translation initiation factor essential for cells according to the present invention And removing the efp encoding the protein elongation factor (EF-P) from the chromosome of the microorganism and introducing the gene expression cassette containing the regulatory gene into the host as Escherichia coli , the plasmid in the antibiotic-free medium without intrinsic mutations between cells. It can be kept stable. In addition, infA in the recombinant microorganism And efp When the expression level is precisely controlled through a promoter, PCN can also be quantitatively controlled with high efficiency, and can be widely applied in various industries to produce recombinant proteins. |
| priorityDate | 2017-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 866.