http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102043603-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0693 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 |
filingDate | 2012-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-102043603-B1 |
titleOfInvention | Isolation of lung cancer cells and proliferation thereof using suspension culture method |
abstract | The present invention relates to a culture condition for effective separation and proliferation of lung cancer cells from the lung cancer tissue of the patient and a screening method of a patient-specific cancer treatment using lung cancer cells cultured by the above method, more specifically 1) from the patient Cutting one lung cancer tissue into a size of 40 to 100 μm in diameter, 2) separating the cut tissue of step 1) into an enzyme by reacting the cut tissue of step 1), and 3) separating the single cell of step 2). And subcultured with the serum-free ACL4 or N2-added medium in the low-attachment culture dish, and 4) subcultured cells using the accutase-produced cells. Step 4) and the method for separating and proliferating lung cancer cells from lung cancer tissue, characterized in that it does not require a separate end of the accutase reaction The present invention relates to a method for screening patient-specific cancer therapeutic agents using lung cancer cells prepared by the method. |
priorityDate | 2012-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 222.