| abstract |
The present invention provides an assay that can be used to predict whether a given ISV will undergo protein interference as described herein and / or will generate a (non-specific) signal in an assay (e.g., an ADA immunoassay) (Such predictive assays may be used, for example, to test whether a given ISV may have such protein interference and / or a tendency to produce such a signal, and / It can be used to select ISVs that are less likely to suffer or less likely to suffer and / or to test whether some change (s) to ISVs will reduce (in whole or in part) their tendency to generate such interference or such signals May be used as an assay or test that may be used, and / or may reduce its tendency to generate such protein interference or signal Which may be used as an assay or test that can be used to guide changes or improvements in the ISV to be effective; A method of altering and / or improving the ISV to eliminate or reduce such protein interference or its tendency to generate such a signal; - changes that can be introduced into the ISV that eliminate or reduce this protein interference or its tendency to generate such signals; ISVs specifically selected for this protein interference or not having a tendency to generate such a signal or having a (lower) / lowered tendency (e.g., by using the assay (s) described herein); And - providing modified and / or improved ISVs that do not have such protein interference or a tendency to generate such signals or that have a (lower) / lowered tendency, and in their specific non-limiting aspects. |