http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101958497-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 |
| filingDate | 2012-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101958497-B1 |
| titleOfInvention | Low fucose cell lines and uses thereof |
| abstract | A method for selecting cells having a zero fucose level useful as a host cell for expressing a recombinant protein is disclosed. The method comprises the steps of: (d) introducing a genetic mutation into the population of CHO cells by contacting the cell with methotrexate (MTX), (e) contacting the cell with a fucose moiety on the cell membrane of the population of cells Contacting a population of CHO cells comprising mutated cells with a non-toxic fucose binding agent for an amount of time that allows binding of the fucose binding agent, wherein the amount of time does not allow killing of the cells; And (f) selecting a cell useful as a host cell for expressing the recombinant protein by removing a partial population of cells that bind the fucose binding agent from a population of cells comprising the mutated cell, wherein the selected cell is a Zero Fucoid Has os content. Cells and cell lines useful as host cells for expressing recombinant proteins are also disclosed. |
| priorityDate | 2011-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 350.