http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101939570-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61D19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-027 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61D19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101939570-B1 |
| titleOfInvention | a production method of a cloned dog and the dog using thereof |
| abstract | The present invention relates to a method for producing duplicates. More specifically, the present invention relates to a method for producing cloned clones characterized by using a donor cell treated with acteoside, and a cloned clone thereof. The present invention relates to a process for isolating and culturing donor cells from canine animals (step 1) A cell culture medium treatment process (step 2) for the donor cells, Preparation of donor eggs for canine (3 courses), The process of producing cloned somatic cells is performed (step 4) (Step 4-1) of removing the cumulus cells around the oocyte of the recipient oocyte, A step of enucleation of the recipient oocytes (step 4-2) (Step 4-3) of injecting the cell culture medium-treated donor cells into the enucleated oocyte, (4-4) of fusing the oocyte cytoplasm with cells by electrophoresis using a needle-like electrode by precipitating couplers injected with the donor cells into a fusion medium, (Step 4-5) of inducing activation by culturing the fused embryos in a porcine conjugate medium (PZM-3) After the cloned fertilized eggs are washed and further cultured in PZM-3 (step 4-6) The cultured somatic cell cloning column is transplanted using the surgical method to the fallopian tube of the surrogate mother (step 4-7) The method comprising the steps of: The present invention also relates to a method for culturing a donor cell in a cell culture medium (step 2) The cells were cultured in DMEM supplemented with 10% (v / v) FBS, 1% penicillin-streptomycin, Or culturing the cells in DMEM supplemented with 0.5% (v / v) FBS, 1% penicillin-streptomycin, Or culturing the cells in DMEM supplemented with 10% (v / v) FBS, 1% penicillin-streptomycin, and culturing the cells by adding acteoside to the culture medium. The present invention also relates to a process for isolating and culturing donor cells from canine animals (step 1) The process of collecting the canine fetus by Cesarean section (1-1), The recovered embryos are transferred to the laboratory to remove the head and limbs and washed with PBS (phosphate buffered saline) (1-2) After the washing, the remaining tissues were minced and cultured in DMEM supplemented with 15% (v / v) FBS (Invitrogen) and 1% penicillin-streptomycin (1-3) (1-4) process of removing the unattached tissue from the culture dish and culturing the adhered cells to confluency, The cultured cells were trypsinized and frozen in liquid nitrogen using FBS to which DMSO was added (1-5) The method comprising the steps of: The present invention also provides a cloned dog produced by the above method. |
| priorityDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 106.