abstract |
The present invention relates to a method for producing a recombinant protein of silkworm storage protein 1 (SP1) using fructose, and more particularly, to a method for producing recombinant SP1 protein produced by cell culture by size exclusion chromatography chromatography, the addition of a certain amount of fructose to the elution buffer suppresses the aggregation phenomenon of the recombinant SP1, and even after concentration through subsequent ultrafiltration, A recombinant SP1 protein produced by the silkworm storage protein 1, a recombinant SP1 protein produced by the silkworm SP1 protein and its inhibition of apoptosis, a cell culture Culture media for the production of biopharmaceuticals, use as anti-oxidation or functional (cosmetic) materials will be. |