abstract |
The present invention relates to primers and probes for simultaneously detecting four subtypes of coronaviruses HKU1, OC43, 229E and NL63, and a method for detecting coronavirus using the same. Specifically, primers and probes capable of simultaneously detecting human coronaviruses HCoV-229E, HCoV-OC43, HCoV-NL63 and HCoV-HKU1 of the present invention are excellent in sensitivity and HCoV-229E, HCoV- -OC43, HCoV-NL63 and HCoV-HKU1 can be specifically detected. Therefore, the primer and probe set of the present invention can be used for detection and diagnosis of HCoV-229E, HCoV-OC43, HCoV-NL63 and HCoV- . |