http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101822902-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 |
filingDate | 2015-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-101822902-B1 |
titleOfInvention | Nuclease-mediated dna assembly |
abstract | A method for producing a complementary end sequence using a sequence-specific nuclease preparation (e.g., a gRNA-Cas complex) and then assembling the overlapping complementary sequences to assemble two or more nucleic acids is described in detail in this specification Lt; / RTI > Nuclease agents (e. G., GRNA-Cas complexes) can generate double strand breaks in dsDNA to generate overlapping end sequences, or generate gaps on each strand to generate complementary extruded end sequences . An assembly using the methods described herein can be constructed by assembling any nucleic acid with overlapping sequences or assembling the sequences without complementary ends using joiner oligos. |
priorityDate | 2014-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 991.