http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101800878-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2217-052 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2010-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101800878-B1 |
| titleOfInvention | Methods for Analyzing a Genotoxicity Using Non-fluorescent Fluorescence Proteins |
| abstract | The present invention provides a method for screening a non-fluorescent fluorescent protein comprising: (a) transforming a fish with a nucleotide sequence encoding a non-fluorescent fluorescent protein having a mutation; (b) treating the transformed fish with the test substance; And (c) measuring fluorescence generated by mutation of the non-fluorescent fluorescent protein into a fluorescent protein by generating a back mutation in the introduced nucleotide sequence in the fish treated with the test substance, And a method for analyzing genotoxicity of a substance. According to the present invention, the MutaFish system, which is a line of fluorescent protein variants of the present invention, preferably a wild-type EGFP mutant transformed with a Brachydanio rerio line, is capable of producing fluorescence (fluorescence) induced by reversion of a fluorescent protein variant It provides an excellent animal system that can produce genetic toxicity of the test substance by producing fry. Thus, the MutaFish system of the present invention can very simply and quickly analyze the genotoxic effects of test substances in eukaryotes, which was a limitation of prior art (e.g., Ames test). |
| priorityDate | 2010-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 148.