http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101796316-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2437 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2010-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101796316-B1 |
| titleOfInvention | Diastatic enzyme from Porodisculus pendulus and method for producing biofeul using same |
| abstract | The present invention relates to a method for producing a biofuel by saccharifying a biomass using a degrading enzyme derived from Porodisculus pendulus and using the same. The optimal culture conditions for the production of glucose - degrading enzyme of P. pendulus and the glycation characteristics of woody biomass were studied. Cellulase and BXL, such as EG, BGL and CBH, were contained in the extracellular enzyme of P. pendulus , and EG and BGL activities were relatively high. The optimum temperature of EG, BGL, and CBH produced in P. pendulus was 50 ℃, but the temperature range with thermal stability was 30 ~ 40 ℃. The optimum pH was 5.5 and the optimum pH in the temperature range of thermal stability was 5.5. Optimal culture conditions of P. pendulus for cellulase production were found to be suitable for increasing cellulase activity by not adding natural cellulose as a carbon source, corn leached powder as a nitrogen source, or a mixture of peptone / yeast extract and vitamins. The optimum concentration of carbon source was found to be 2% (w / w), the optimum culture pH and temperature were 5.5 ~ 6.0 and 25 ~ 30 ℃. As a result of cultivating P. pendulus and concentrating it 40 times, EG was 3670.5 U / ml, BGL and CBH were 631.9 U / ml, 398.5 U / ml, BXL was 15.2 U / ml, And showed very high enzyme activity. The Filter Paper Unit of the same enzyme was also significantly higher at 11 FPU / ml. P. pendulus obtained under optimal culture conditions As a result of glycation test on various substrates with enzymes, low glycation rate was shown for untreated hardwoods. However, in the case of natural cellulose (Aldrich, ~ 20 micron) and rice straw, high saccharification performance of 50.5% and 33.1% Respectively. This glycation level is about 110% of Celluclast 1.5L, a commercial enzyme derived from Trichoderma reesei , when compared with the same enzyme concentration (30 FPU / g, glucan). Through the mass production technology development process, Of the strains were highly likely to be commercialized. |
| priorityDate | 2010-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 152.