http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101794298-B1
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2009-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-101794298-B1 |
titleOfInvention | Compositions and methods for the assembly of polynuceleotides |
abstract | The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of polynucleotide components. The method of the present invention comprises the steps of annealing a linker sequence, annealing linker sequence pairs LA and LB, or annealing linker sequence / primer binding fragment pair LA and PB, or DNA fragment D Lt; / RTI > D-LB, LA-D-LB and LA-D-PB or D-LB, LA-D-LB and LA- RTI ID = 0.0 > D < / RTI > components. The sequence of the linker sequences LA and LB that can be annealed provides a complementary terminus to the DNA fragment, which can be used in host cell mediated homologous recombination or in the assembly of one or more of the various DNA segments ) Polymerase for aligned aggregates to polynucleotides Used in conjunction with primers that bind fragment PA and PB in a cyclic aggregation reaction. |
priorityDate | 2008-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 941.