| abstract |
The present invention provides a chromatographic separation process for recovering polyunsaturated fatty acid (PUFA) products from a feed mixture comprising: (a) using a mixture of water and a first organic solvent as the eluent to obtain an intermediate product Thereby purifying the feed mixture in a first chromatographic separation step; And (b) purifying said intermediate product in a second chromatographic separation step, using a mixture of water and a second organic solvent as eluent, to obtain a PUFA product, wherein said second organic solvent is 1 organic solvent and has a polarity index of the first organic solvent and a polarity index different by between 0.1 and 2.0 wherein the PUFA product is selected from the group consisting of alpha-linolenic acid (ALA), gamma-linolenic acid (GLA), linoleic acid, Di- or triglycerides, GLA mono-, di- or triglycerides, linoleic acid mono-, di- or triglycerides, ALA C 1 -C 4 alkyl esters, GLA C 1 -C 4 alkyl esters or linoleic acid C 1 -C 4 alkyl ester or a mixture thereof. |