abstract |
The present invention relates to a method for obtaining peripheral blood mononuclear cells from peripheral blood; CDK antibody is cultured in the culture medium in a culture medium containing IL-2, CD3, CD16, CD56, and CD335 antibodies, and a step of pre-coating the CD16 antibody in a culture container, The present invention relates to a method for mass proliferation of NK cells having a cell ratio of 70% or more. According to the culturing method of the present invention, the medium containing IL-2 and CD16, CD56, and CD335 can be selectively differentiated into NK cells, NKT cells, T cells, etc. In particular, Or more. |