| abstract |
The present invention relates to polypeptides having aldolase activity, including, but not limited to, HMG and KHG aldolase activity, polynucleotides encoding such polypeptides, and polypeptides and polynucleotides, And a method of using the same. In some embodiments, the invention provides pyruvate aldolase activity, including, but not limited to, thermal safety or heat-resistant activity, polypeptides having aldolase activity, including HMG and KHG aldolase activity, Polynucleotides, and methods of making and using the polypeptides and polynucleotides. The polypeptides according to the present invention may be useful in a variety of pharmaceutical, industrial, and / or agricultural applications. In some embodiments, the present invention relates to the use of R-2-hydroxy 2- (indol-3ylmethyl) -4-keto glutaric acid (R-MP), certain stereoisomers of monatin, S, R monatin, and salts thereof, as well as polypeptides and biosynthetic pathways useful for producing certain stereoisomers of monatin derivatives, such as the R, R and S, R sequences and salts thereof. |