http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101650869-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-035 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 |
| filingDate | 2012-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101650869-B1 |
| titleOfInvention | Method for highly expressing recombinant protein of engineering bacteria and use thereof |
| abstract | A method for high-efficiency expression of an engineering bacterial recombinant protein and its application are provided. (1) of obtaining a positive monoclonal colony by transfecting a recombinant mutant plasmid into an E. coli pET system engineering bacterium; (2) a step of culturing a positive single clonal colony to obtain a seed broth and inducing the seed broth to grow by mass-multiplication; The method comprising (3) isolating the cell supernatant containing the expressed recombinant protein and purifying the recombinant protein of the cell supernatant is characterized in that the E. coli pET system engineering bacteria means E. coli B834 (DE3) . In addition, the components and protein scouring steps of the bulk enrichment medium have been optimized, resulting in significant improvements in protein yield and purity, and E. coli engineering bacteria are applied to scale production for use in expressing recombinant proteins. |
| priorityDate | 2011-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.