http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101639650-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-06 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2012-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101639650-B1 |
| titleOfInvention | Method for separating and purifying rna from biomaterial |
| abstract | The present invention provides a method for separating pure RNA from a biological material. The present invention relates to a method for producing a dehydrated biological sample, comprising the steps of: (1) mixing a tissue organs with an aqueous solution of a monovalent cationic salt to obtain a dehydrated biological sample; (2) mixing and hatching a dehydrated biological sample with a monovalent cationic salt aqueous solution; (3) Adding, mixing, and centrifuging the aqueous solution of monovalent cationic salt to precipitate, pouring the supernatant into another centrifuge tube; And (4) isopropyl alcohol are mixed and centrifuged to discard residual impurities visible between the supernatant liquid, the bed liquid and the upper and lower phases to obtain a white RNA precipitate. The method of the present invention can efficiently remove the protein of the RNA in the biological sample to obtain a pure RNA product so that the protein remaining in the product can avoid the decomposition action on the product RNA; The reagents used by the present invention are low toxicity compounds and are very low in environmental and human risk; In addition, the operation is simple, the work is possible without the technical expertise of the staff, the equipment is simple, the cost is low, and the RNA yield and purity are both very high. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190059523-A |
| priorityDate | 2011-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 127.