abstract |
The present application provides a method for identifying a protease with modified substrate specificity or other properties. The method comprises contacting a substrate with a protease such as a serine, alpha macroglobulin or p35 family protein or modified serine and a modified p35 family member or modified alpha- macroglobulin that captures the protease by forming a stable complex upon cleavage of the substrate Thereby screening the treasure and the modified protease. Also provided herein are modified proteases.n n n n Protease screening methods, protease capture polypeptides, substrate specificity, modified proteases, |