| abstract |
The present invention relates to a multi-stage cell culture structure having a three-dimensional structure, an assay chip containing the same, and a cell analysis method using an assay chip, More specifically, it includes at least two cell culture layers including a mat-like cell support of a three-dimensional structure formed of hybrid nanofibers having a nanometer-micrometer diameter, and at least one cell culture layer containing one type The present invention relates to a multi-stage cell culture construct having a three-dimensional structure and an essence comprising the same, characterized in that the cultured cells are infiltrated or transferred into a cell support of a culture layer or other culture layer into which cells are injected, A chip and a cell analysis method using the above-mentioned assay chip are disclosed. According to the present invention, a cell culture construct is constituted by at least two cell culture layers, allowing movement through a three-dimensional space between cells, and each cell culture layer is formed of hybrid nanofibers having a nanometer-micrometer diameter Based on the matted cell support of the three-dimensional structure, three-dimensional cell culture and cell migration are possible, and the cells can be measured and analyzed in real time. |