http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101448353-B1
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 |
filingDate | 2012-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2014-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-101448353-B1 |
titleOfInvention | Method for analyzing the protease activity and the application thereof |
abstract | The present invention relates to a method for analyzing proteolytic enzyme activity, comprising the steps of: introducing a modified portion containing a carboxyl group at one end to the surface of metal nanoparticles to modify the surface of the metal nanoparticles to a carboxyl group; Preparing a connection portion into which polyhistidine is introduced at both ends of a polypeptide substrate that is specifically degraded by the protease to be analyzed; Aggregating the metal nanoparticles into which the carboxyl group has been introduced as an experimental group, in a buffer solution containing a divalent metal cation, an analyte to be analyzed and the connecting portion; Measuring the degree of aggregation of the metal nanoparticles; And comparing the measured degree of aggregation with the degree of aggregation of metal nanoparticles in a control group aggregated in a buffer solution containing no protease to be analyzed. The method for analyzing protease activity of the present invention can analyze the activity of protease in a simpler, less expensive and faster manner than the conventional technique, and is useful for screening protease activity inhibitor screening method and diagnosis of protease-mediated diseases Lt; / RTI > |
priorityDate | 2012-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 263.