| abstract |
The present invention relates to the preparation of antibodies and the use thereof of the working heavy chain of various repertoires to effect affinity maturation. The present invention also relates to the preparation and use of group-specific heavy chain antibodies of various repertoires and to the use of multivalent polypeptides with antibody heavy chain functionalities, preferably antibody heavy chain binding functionalities, constant region effector activity and optionally further effector functions ≪ / RTI > composites. The invention also relates to a method of producing an antibody of the working heavy chain only in transgenic mice that respond to antigen challenge. In particular, the present invention relates to the production of a mixture of human antibody-specific, high affinity heavy chain antibodies or groups of all groups and a method for isolation and expression of a fully functional V H antigen binding region. |