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filingDate 2007-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2014-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-101390971-B1
titleOfInvention Production method of smallpox live vaccine
abstract The present invention provides a safe smallpox vaccine and a method for its production when the content of MSP is suppressed to a low level. A step of inoculating and culturing the master seed solution of the attenuated vaccinia virus into a suitable number of the rabbit kidney cells (1 to n: n is an integer), inoculating a part of the culture solution of each group into RK-13 cells and Vero cells A step of selecting and collecting a culture medium of each group which does not generate plaque in Vero cells, a step of producing a vaccine stock solution by using the culture solution (working seed solution), and a step of culturing the RK- To a process for producing a smallpox vaccine, and to a smallpox vaccine obtained by such a method.
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