http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101349070-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2012-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101349070-B1 |
| titleOfInvention | Foreign gene coexpression vector |
| abstract | The present invention relates to a foreign gene forced expression vector, specifically, T7 promoter, lactose operator and three-frame Simultaneous Translation (TFST) construct [three ribosomal binding sites (RBS) and Three start codons (ATG) -containing fragments which are started in different frames] were sequentially constructed, and a gene-forced expression cassette including one or two gene-expression cassettes was introduced into the vector. A DNA fragment to be cloned is transcribed into one transcription unit (mRNA), from which a forced expression vector can be produced from which three or six frames of polypeptide can be translated simultaneously, and the green fluorescence in three or six frames on the vector. After cloning the protein gene, each recombinant expression vector obtained was high in all the green fluorescent protein in the transformed strain. Since it was confirmed that the expression level, the novel expression vector of the present invention can be used for the production of metagenome, genome, or cDNA library, as well as a method for expressing the active protein by preparing a metagenomic library, genomic library or cDNA library The present invention can be useful for screening useful genes from these libraries, or for discovering genes using an in vitro transcription / translation system. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019245066-A1 |
| priorityDate | 2011-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.