| abstract |
The present invention relates to a method for mass purification of silkworm hemolymph-derived proteins and proteins purified therefrom. In the mass purification method of silkworm hemolymph-derived protein of the present invention, a first step of producing a silkworm dried material by lyophilizing silkworm, a second step of removing the seal gland from the silkworm dry material to obtain the remaining blood lymphocytes, grinding the hemolymph And then adding water to heat treatment to prepare hemolymph lysate, a fourth step of centrifuging the hemolymph lysate to obtain a supernatant, and a fifth step of separating the 70 kDa protein from the obtained supernatant through a column. Steps. According to the present invention, silk hemolymphs such as cell cryopreservatives or cell culture medium additives can be utilized for various purposes, and in particular, the components remaining after separation of silkworm hemolymphs can be recycled, thereby deriving silkworm hemolymphs, which contribute to the income increase of sheep farmers. Provided are a method for mass purification of proteins and purified proteins therefrom. |