http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101337408-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8209 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-06 |
| filingDate | 2010-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101337408-B1 |
| titleOfInvention | Improved Agrobacterium Transformation Conditions for Efficient Production of Transgenic Symbidium Plants |
| abstract | The present invention relates to the production of highly efficient transformed symbidium plants through immersion treatment, inoculation concentration, co-culture period, and acetosyringone addition in symbidium progeny in symbidium transformation using Agrobacterium. An object of the present invention is to provide a method that can be effectively applied to the production of transformed symbidium plants using Agrobacterium. In detail, when inoculating Agrobacterium into the protoplast (PLB) fragment used for transforming symbidium, optimal culture inoculation is performed by varying the immersion treatment and inoculation concentration so that Agrobacterium can be infected by contacting plant cells. The purpose of this study is to find the concentration and to provide the optimal method for transforming Agrobacterium symbidium through the exploration of the optimum incubation period and acetosyringone addition effect through treatment of various co-culture periods and the addition of acetosyringone. In order to achieve the above object, the present invention is to increase the probability that the optimal Agrobacteria culture medium can be contacted and infected with plant cells through immersion treatment and various inoculation concentrations in symbidium transformation using Agrobacterium, After infection, it is characterized by consisting of a step of finding the optimal culture period when co-cultivation of plant cells and Agrobacterium, and finally by adding acetosyringone during the co-culture. The effect of the present invention is to provide a highly efficient method for Agrobacterium mediated transformation using the Symbidium protoplasts as fragments. More specifically, it provides an effective method of infection by investigating the effects of immersion treatment and inoculation concentration on the first Agrobacterium infection in symbiotic protoplast (PLB) tissues, and then optimizes the culture period and aceto in co-culture with plant cells. Investigating the effect of the addition of cylingon provides a more efficient transformation method. [Index] Symbidium, agrobacterium, prokaryotes, transformation, |
| priorityDate | 2010-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.