http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101257002-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-105 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-10041 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 |
| filingDate | 2010-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101257002-B1 |
| titleOfInvention | Method of manufacturing a transgenic animal using ultrasonic imaging based gene transfer technology |
| abstract | The present invention relates to a method for producing a transgenic animal using Ultrasound Image-guided Gene Delivery (UIGD) technology, specifically E8.5 to E9.5 by delivering a gene based on the ultrasound image Genes can be injected into the ventricle of mouse embryos at an early stage of development, and mouse leukemia virus (MLV) -based retroviral vectors, or 3'LTRs thereof, are known as Murine Stem Cell Virus (MSCV). The retroviral vector substituted with 3'LTR is capable of being produced at high concentration due to improved viral titer, a MLV-based retroviral vector having a gene encoding the target protein and MSCV 3'LTR, and a bullous stomatitis virus-G (vescular) By injecting a retrovirus prepared using stomatitis virus (VSV-G) protein as an envelope protein into the ventricles of mouse embryos, Since the protein was stably expressed in early embryonic mouse embryos and adult brain cells, the optimized UIGD technology and gene delivery system can be usefully used for the preparation of transgenic animals expressing brain cells specificly. have. |
| priorityDate | 2010-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 207.