abstract |
Methods of making immune system molecules, particularly humanized antibodies, and fragments thereof are disclosed. The general method helps to identify similar human framework regions suitable for making molecules by optimizing the similarity between individual antibody framework regions. Avoid comparisons of larger antibody frameworks, hypervariable domains, or both. Also disclosed are humanized antibodies by the method. The present invention has a wide range of applications, including use in the production of humanized monoclonal antibodies with suitable binding affinity and minimized human immunogenicity. |