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filingDate 2011-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2012-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2012-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-101188714-B1
titleOfInvention Method for simultaneous determination of nucleotides in plasma with capillary electrophoresis-mass spectrometry
abstract PURPOSE: A method for simultaneously detecting nucleotides in blood plasma using a capillary electrophoresis-mass spectrometer is provided to effectively separate nine kinds of nucleotides from a blood plasma specimen by securing optimal analyzing conditions of the capillary electrophoresis-mass spectrometer. CONSTITUTION: A method for simultaneously detecting nucleotides in blood plasma includes the following: protein is removed from the blood plasma; the blood plasma without the protein passes through a micelle electrokinetically capillary chromatography column which is adjusted with a surfactant containing developing solvent; and the treated blood plasma is analyzed by a capillary electrophoresis-mass spectrometer. The protein is removed using 50-70% of an ethanol solution containing ethylene diamine tetraacetic acid(EDTA). The concentration of the EDTA is in a range between 1 and 5 mM. The surfactant is a cationic surfactant. The developing solvent is an ammonium acetate buffer solution. The concentration of the ammonium acetate buffer solution is in a range between 10 and 60 nM.
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