http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101171434-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B61-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2445 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2009-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2012-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2012-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-101171434-B1 |
| titleOfInvention | Discovery and improvement of isatin synthase and natural indirubin production method combined with beta-glucosidase |
| abstract | The present invention relates to a method for producing indirubin, which is used as a dye, a pharmaceutical raw material, and a physiologically active substance by acting on an indican contained in a natural plant, beta-glucosidase (β- The present invention provides a method for producing a large amount of indirubin by transforming microorganisms including genes encoding glucosidase and isatin generating enzyme.n n n More specifically, the present invention relates to the discovery and improvement of the oxygenase that exhibits selective oxidative activity at the C-2 position of the indoksil during the preparation of indirubin, registered in GenBank by bioinformatics The functional oxygena selected by searching for and extracting the relevant gene from the sequence and inserting the transformed recombinant strain into the LB solid medium containing ampicillin and indican to obtain a reddish clone. It is to provide an agent and a preparation method thereof.n n n In addition, after transforming the recombinant gene produced by random mutagenesis to increase the activity of the discovered oxygenase to a recombinant strain containing glucosidase, a clone containing a gene with an increased indyrubin-producing ability compared to the control was obtained. It is to provide a method for producing indirubin using the improved genetic resources as a process.n n n n Oxygenase, Glucosidase, Indican, Indoksil, Isatan, Indirubin |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210150829-A |
| priorityDate | 2009-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1070.