http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101149418-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate | 2009-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2012-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2012-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-101149418-B1 |
titleOfInvention | How to measure bacteria |
abstract | The present invention relates to a method for measuring the amount of bacteria using a fluorescent nanoparticle conjugate, an object of the present invention, by using the intensity of the fluorescence emitted from the fluorescent nanoparticle conjugate attached to the bacteria to determine the amount of bacteria It is to provide a bacterial amount measuring method that can be measured. To this end, the present invention, the first step of separating the specific bacterial population which is a collection of specific bacteria among the different bacteria formed on the sample, respectively; As the fluorescent nanoparticle conjugate containing the antibody against the specific bacteria belonging to the specific bacterial population is introduced into the specific bacterial population, the specific bacteria is attached to the fluorescent nanoparticle conjugate and the fluorescent nanoparticle conjugate A second step of labeling specific bacteria attached thereto; A third step of removing the specific bacteria and the unattached fluorescent nanoparticle conjugate; And the amount of fluorescence detected from the fluorescent nanoparticle combination attached to the specific bacterium is measured by a pre-installed bacterial volume measuring device. And a four step, wherein the fluorescent nanoparticle conjugate is formed by forming a biomolecule avidin layer on the surface of the fluorescent nanoparticle (QD) having 10 to 80 nmol and then reacting the biotin-coupled antibody to the avidin layer. In the second step, the attachment process between the specific bacteria and the fluorescent nanoparticle conjugate is performed by the specific bacteria reacting with the biotin-bound antibody first and the avidin layer reacting with the biotin. do.n n n n Fluorescent Nanoparticles, Bacteria, Fluorescence |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014030985-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10281461-B2 |
priorityDate | 2009-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.