| abstract |
The present invention relates to a method of inducing differentiation into insulin secretory cells using human adult stem cells, and more particularly, to induce differentiation of stem cells isolated from subcutaneous adipose tissue into insulin secreting cells having excellent insulin secretion efficiency. As a method, the glucose concentration in the culture was sequentially processed from high concentration to low concentration, and the culture medium was supplemented with B27 supplement, fibroblast growth factor-2, epidermal growth factor and nicotine. Cytokine, such as nicotinamide, glucagon-like peptide-1, Activin A, insulin-like growth factor, and betacellulin Differentiation into insulin secreting cells, a cell therapy for the treatment of type 1 diabetes, by using a culture method that treats growth factors in two or four stages Doha is on the way.n n n n Induction of differentiation into stem cells, insulin secreting cells, subcutaneous adipose tissue, cytokines, growth factors |