| abstract |
The present invention is modified to be further modified by heterologous expression of a series of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of glycoproteins for the treatment of mammals such as humans. A host cell has a lipid-binding oligosaccharide. The method provides a designed host cell that can be used to express and target any given gene (s) involved in glycosylation. Host cells with modified lipid-binding oligosaccharides are prepared or selected. N-glycans prepared in the designed host cells can be further modified with heterologous expression of one or more enzymes, such as glycosyltransferase, sugar transporters and mannosidase, resulting in GlcNAcMan 3 GlcNAc producing human-like glycoproteins. It has a two core structure. For the production of therapeutic proteins, the method will be applied to design cell lines from which any desired glycosylation structure can be obtained. |