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filingDate 2008-01-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2010-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-100965812-B1
titleOfInvention Extraction of growth points in scales during garlic growth and production of seed ball by cultivation
abstract For the production of garlic seedlings, garlic plants are prepared during the scale period of garlic in the growing season. Peel off the outer leaves from the garlic plants, cut the roots, cut the lower part containing the diameter and prepare the sample, sterilize the sample, dry it, extract the growth point from the sterilized sample, and then grow the growth point on the medium for a period of time. It is cultured on board to produce globules. In order to produce the globules, the growth point is cultured in a first medium to which growth regulators containing 0.1 to 1.0 mg / L NAA and 1 to 5 mg / L 2ip are added to LS basal medium. Thereafter, the plants cultured in the first medium are first subcultured in a second embryo in which the sucrose content in the first medium is increased. Thereafter, the plants cultured in the second medium are second subcultured in the third medium except for the growth regulator in the second medium. At this time, the sucrose content of the first medium is 25 ~ 35 g / L, the sucrose content of the second medium is 65 ~ 75 g / L. After incubating the growth point in the medium, the incubation period takes about 80 to 90 days. As such, if the growth point is extracted and cultured in the scale of the garlic growing period, the extraction is easy and simple, so that mass culture is possible, and the in-flight cultivation period is shortened by 1.5 months or more than the existing method, so that the production of in-flight plants and small bulbs becomes easy.
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