http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100963014-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-02027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-531 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 |
| filingDate | 2007-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2010-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100963014-B1 |
| titleOfInvention | Method for preparing novel low-temperature uracil-DNA glycosylase derived from Bacillus spysis НＪ171 strain and its use in polymerase chain reaction |
| abstract | The present invention relates to uracil-DNA glycosylase (UDG) derived from Bacillus sp. HJ171 strain and its use, and more particularly to low-temperature uracil- isolated from Bacillus sp. HJ171 ( Bacillus sp. HJ171). Gene of DNA glycosylase and amino acid sequence inferred therefrom, expression and purification in Escherichia coli of Bsp HJ171 uracil-DNA glycosylase (UDG) gene, biochemistry of UDG produced thereby Properties and its use in polymerase chain reaction (PCR).n n n The uracil-DNA glycosylase (UDG) of the present invention has an activity of specifically degrading uracil bases in the DNA substrate containing uracil at low temperatures and easily denatures at high temperatures, thus occurring during PCR using dUTP. Carry-over contamination can be easily removed. Therefore, the novel Bsp HJ171 uracil-DNA glycosylase (UDG) of the present invention can be effectively used to increase the accuracy, elimination of false positives, ie purity and amplification efficiency of PCR in clinical diagnosis using PCR. have.n n n n Bacillus spysis HJ171, uracil-DNA glycosylase (UDG), polymerase chain reaction (PCR) |
| priorityDate | 2007-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 319.