http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100863283-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-79 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2002-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2008-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2008-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100863283-B1 |
| titleOfInvention | Observation method of intracellular process and recombinant genes used therefor |
| abstract | The present invention relates to a method for selectively confirming specific characteristics related to the movement of a specific protein and the formation of a spatial distribution in a cell through fluorescence images, and to a recombinant gene used in the method. The method comprises the steps of: (a) a recombinant gene wherein one or more genes encoding a signal protein comprising a moiety controlling a positional movement into a specific organelle in the cell and a gene encoding a fluorescent protein representing fluorescence are respectively connected; Preparing a; (b) preparing a hybrid plasmid comprising a promoter portion and a terminator portion so that the recombinant gene can be expressed in a cell; (c) preparing a transformed cell by injecting one or two or more hybrid plasmids prepared in step (b) into the cells; (d) expressing a reporter protein linked with a signal protein and a fluorescent protein in the transformed cell; And (e) observing the distribution of the reporter protein expressed in the transformed cells through fluorescence imaging during, during, or after expression.n n n n Transformation, intracellular organelles, signaling proteins, fluorescent proteins, reporter proteins, trafficking |
| priorityDate | 2002-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 104.