| abstract |
The present invention relates to the field of thrombolysis and the preparation of tissue plasminogen activator (tPA) derivatives in prokaryotic cells. The present invention relates to a method for preparing recombinant DNA-derived tPA, variants thereof, or (Kringle 2 serine) K2S molecules or variants thereof in prokaryotic cells, wherein the tPA or K2S or variants Secreted extracellularly as a correctly folded, active protein, the prokaryotic cell contains and expresses a vector comprising the DNA encoding the tPA or K2S or variant operably linked to a DNA encoding signal peptide OmpA. The present invention also relates to specific K2S derivatives obtainable by the above method. The invention also relates to the use of said DNA molecule and said DNA molecule in said method. n n n n Thrombolytic, tissue plasminogen activator, Kringle 2 serine protease, signal peptide OmpA, prokaryote |