http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100840621-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01017 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1136 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2462 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2005-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2008-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2008-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100840621-B1 |
| titleOfInvention | Method for preparing protein-polymer conjugate |
| abstract | Disclosed is a method for preparing a protein-polymer conjugate, which simplifies a multi-step process including a protein refolding process and a PEGylation process, thereby greatly improving process efficiency. The method for preparing a protein-polymer conjugate may include: crushing a host cell including a protein produced in the form of an inclusion body, centrifuging, and adding a denaturant to the obtained inclusion body pellet to unfold the aggregated protein; Introducing the inclusion body lysate including the denaturing agent and the released protein into a column to adsorb the released protein to a resin filled in the column; Passing an activated water soluble polymer solution through the column to bond the polymer to a protein adsorbed on the resin to produce a protein-polymer conjugate; Passing a washing buffer through the column to remove unreacted activated water soluble polymer solution and refolding the adsorbed protein-polymer conjugate into the refold buffer; A method of preparing a protein-polymer conjugate comprising the step of obtaining a protein-polymer conjugate refolded by the refold buffer from the column.n n n n Lysozyme, Refold, Protein-Polymer Conjugate, Polyethyleneglycol (PEG) |
| priorityDate | 2005-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 181.