abstract |
The present inventionn n n a) a plasmid containing at least one nucleotide sequence encoding a pathogen immunogen of the animal species of interest under conditions capable of in vivo expression; Andn n n b) cationic lipids containing quaternary ammonium salts of the general formula:n n n n n n n n Wherein R 1 is a saturated or unsaturated linear aliphatic radical containing 12 to 18 carbon atoms, R 2 is another aliphatic radical containing 2 or 3 carbon atoms, and X is a hydroxy group or Amine group, the lipid is preferably DMRIE)n n n It relates to a DNA vaccine against a pathogen for attacking a production breeding animal, particularly a cow or a pig comprising a.n n n Vaccine, nucleotide, plasmid, vector, intron, immunization, breeding animal, cattle, pig |