http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100803384-B1

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grantDate 2008-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2008-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-100803384-B1
titleOfInvention How long-term storage of skin tissue
abstract Storage method of the skin tissue and dermal skin dermal layer for transplantation of the present invention comprises a primary glycerol solution treatment step of immersing and stirring the glycerol solution of 50% concentration at room temperature after washing the skin tissue extracted from the skin tissue donor; A secondary glycerol solution treatment step of immersing the primary glycerol solution treated skin tissue in a glycerol solution of 70% concentration at room temperature and then incubating the mixture; And a tertiary glycerol solution treatment step of immersing the secondary glycerol solution treated skin tissue in a glycerol solution that maintains a concentration of 85% at room temperature, and then continuing to store the sample at 4 ° C. to stabilize the tissue. .n n n All of the stored skin tissue and graft-free dermal layers in this way are replaced with glycerol solutions instead of water from the tissues, and thus the shape and function of various components, including proteins / collagens, that can reconstruct the skin during transplantation. The basal membrane, which is well maintained and corrects for the reattachment of living endothelial cells or epithelial cells, can be provided intact and the tissue intact can be maintained without structural changes and damage.n n n In addition, antibiotics contained in glycerol and solution inactivate the cells that cause immunorejection reactions, and remove moisture to inhibit bacterial contamination, making it easy to apply to transplant patients and easy to store and transport.
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