| abstract |
It is an object of the present invention to improve the efficacy and protective effects induced by DNA vaccination against Paramyxoviridae virus and herpes virus in pets and sport animals. Improvements in DNA vaccination include formulation of vaccines with cationic lipids containing a quaternary ammonium salt, DMRIE, or modification of the nucleotide sequence encoding the antigen of interest, more specifically the nucleotide sequence fragment encoding the transmembrane domain of the antigen of interest. Deletion and / or insertion of a nucleotide sequence encoding a signal peptide and / or insertion of an intron, or addition of GM-CSF, or a combination thereof. The invention also relates to a vaccine obtained thereby.n n n n Vaccine, nucleotide, plasmid, vector, intron, immunization, pet, sport animal |