http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100762261-B1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2001-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2007-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2007-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-100762261-B1 |
titleOfInvention | Full length complementary deoxyribonucleic acid production method and anchors and primers used therein |
abstract | The present invention relates to a method for preparing full-length complementary deoxyribonucleic acid (hereinafter, abbreviated as cDNA), and more specifically, by acting a reverse transcriptase enzyme on messenger ribonucleic acid (hereinafter, referred to as mRNA). 3 to 4 deoxycytidine monophosphate (dCMP) group is a complex of the cDNA strand and mRNA is coupled to the 3 'end, and the biotin or phosphate group is coupled to the 3' end and the phosphate group is bonded to the 5 'end Adenylating the single stranded anchor; Selecting the full-length cDNA / mRNA complex by selectively binding the adenylated single-strand anchor to the 3 'end of the cDNA strand of the cDNA / mRNA complex; It relates to a selective amplification method of the full-length cDNA comprising the step of proceeding the polymerase chain reaction (Polymerase Chain Reaction) using the full-length cDNA / mRNA as a template using a primer specifically binding to the anchor.n n n n Complementary deoxyribonucleic acid |
priorityDate | 2001-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 252.