http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100719389-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-775 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2005-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2007-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2007-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100719389-B1 |
| titleOfInvention | Method for isolating and purifying apolipoprotein A-I from human plasma |
| abstract | The present invention relates to a method for separating and purifying apolipoprotein AI from human plasma, wherein cohn's fraction IV paste fractionated from human plasma is urea, alcohol, guanidine hydrochloride. dissolving in guanidine hydrochloride or a mixture extract thereof, and then removing the filter aid to obtain a supernatant by centrifugation or filtration. A primary impurity removal step of adding PEG (polyethylene glycol) to the supernatant and stirring to precipitate plasma protein impurities of high molecular weight, followed by centrifugation or filtration to obtain a supernatant; A secondary impurity removal step of precipitating plasma protein impurities of high molecular weight by mixing alcohols having an alcohol concentration of 50 to 70% in the supernatant and stirring them at -10 to 10 ° C; A third impurity removal step of heating and cooling the cold alcohol-treated mixture and centrifuging or filtration to obtain a supernatant; Gel-filtration or dialysis the supernatant to remove alcohol; And a lipid removal step of removing lipid components through an ion exchange column to provide high purity apolipoprotein A-I.n n n n Apolipoprotein A-I, plasma, cohn's fraction method, corn fraction IV, cold ethanol method, heat treatment |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100992488-B1 |
| priorityDate | 2005-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 175.