http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100707964-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-72 |
| filingDate | 2004-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2007-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2007-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100707964-B1 |
| titleOfInvention | A method for recovering recombinant proteins by incorporating a recombinant protein expression vector containing P.S promoter and genes encoding the choline and endoricin causing cell destruction into E. coli and culturing in a medium containing glucose |
| abstract | The present invention provides a gene encoding E. coli and a gene encoding a choline derived from T4 bacteriophage that disrupts the cell membrane of Escherichia coli at the origin of replication, MCS, selection marker, and the gene sequence shown in SEQ ID NO: 2. Constructing a recombinant vector by inserting a gene encoding a foreign protein into a vector containing a gene encoding endoricin derived from T7 bacteriophage that destroys the cell wall of the cell (a); (B) transforming Escherichia coli by inserting the recombinant vector of step (a) into Escherichia coli; (C) culturing the transformed Escherichia coli in step (b) in a medium to which glucose is added; And, after the cultivation, the step (c) recovering the foreign protein from the medium comprising the step of recovering the foreign protein, characterized in that the foreign protein is recovered in large quantities by discharging the foreign protein outside the host And useful for screening.n n n n ptsG promoter, holin, endoricin, vector, recombinant protein |
| priorityDate | 2004-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 318.