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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A23L33-10
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-00
filingDate 2005-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2006-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0d2e4be563cc9f6ecc020fe6f513fc4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eded91daa10f4114d45f3e538d7e2683
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publicationDate 2006-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-100596045-B1
titleOfInvention Method for preparing ethanol extracts of larvae
abstract The present invention provides a method for preparing an ethanol extract of Halocynthia roretzi, comprising the following steps: a) Collecting worms in clean waters of the southeast coast of Korea, washing them in distilled water 5-6 times, ), And then dried at 35 to 50 ° C. for 3 to 4 days; b) pulverizing the dried squid with 70 to 90 mesh, injecting 400 to 600 g of distilled water per 100 g of the squirted squid, and then hot water for 3 to 6 hours until the reaction solution becomes 100 g; c) The heated reaction solution was placed in a round flask equipped with a reflux condenser, and 500 ml of 75% ethanol was added three times for 8 to 9 hours in a water bath at 75 to 100 ° C., followed by extraction, filtration and air drying. ; d) 1.0 g of the product of step c) was diluted and diluted with a mixed solvent of distilled water and dimethyl sulfoxide (DMSO) mixed at a ratio of 10: 1 to 20: 1, at a concentration of 45-65 μg / μl. Obtaining a sample solution of; And e) repeating steps a) to d) to form a sample solution at a concentration of 800 to 1,200 μg / μl, and then sterilizing in an autoclave at 100 to 130 ° C.n n n The unglong ethanol extract prepared according to the method of the present invention contains herbal ingredients such as chromium, synthol, chondroitin sulfate, ribose, deoxyribose, cellulose and the like, and these herbal ingredients are HeLa tumor cells As well as anti-cancer effects against E. coli, Staphylococcus aureus, Klebsiella pneumoniae, Bacillus subtilis, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella paratiphi, Shigella sonnei, Yersi Excellent antibacterial effect on bacteria in the digestive tract and intestinal tract, such as nia entercholica.n n n n Halocynthia roretzi, ethanol extract, HeLa tumor cell.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101274868-B1
priorityDate 2005-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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