http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100562873-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2003-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2006-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2006-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100562873-B1 |
| titleOfInvention | Human insulin expression plasmid and method for preparing human insulin using the same |
| abstract | The present invention relates to a human insulin expression plasmid and a method for preparing insulin using the same.n n n The plasmid of the present invention comprises a nucleotide sequence encoding the compound of the R-B-X-A structure.n n n In the above structure, R is a full-sequence peptide consisting of Met-Thr-Met-Ile-Thr-Y, Y is lysine, arginine, a peptide whose C-terminal amino acid is lysine, or a C-terminal amino acid is arginine Is one of the peptides, B is a human insulin B chain or an analog thereof, X is a peptide connecting B and A, and A is a human insulin A chain or analog thereof.n n n The method for preparing insulin using the plasmid of the present invention converts the proinsulin fusion protein to human insulin by one enzymatic cleavage process, and also minimizes the formation of by-products following enzymatic cleavage, thereby obtaining insulin in high yield.n n n Therefore, the plasmid of the present invention and the method for preparing insulin using the same can be usefully used for industrial mass production of human insulin. |
| priorityDate | 2002-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 375.