| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S424-826
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-525
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S424-816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2720-10051 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-14 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12 |
| filingDate |
1997-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2005-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2005-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-100475427-B1 |
| titleOfInvention |
A method for generating birnavirus from synthetic rna transcripts |
| abstract |
By using synthetic transcripts derived from cloned DNA, a system has been developed for producing live bernaviruses, such as the infectious bursa disease virus (IBDV), a segmented double-stranded (ds) RNA virus of the Birnavirdae family. Separate full-length cDNA clones were constructed that contain the entire coding and non-coding regions of RNA segments A and B of IBDV, respectively. Synthetic RNA of both segments was prepared by in vitro transcription of the linear plasmid using T7 RNA polymerase. Infectious virus was produced 36 hours after transduction by transducing Vero cells with bound plus-sense transcripts of both segments. The development of reverse genetics systems for dsRNA viruses would be of great help in the study of viral gene expression control, pathogenesis, and the design of new methods of making live inactivated vaccines. |
| priorityDate |
1996-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |