http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100461577-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-626 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-62 |
| filingDate | 2002-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2004-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2004-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100461577-B1 |
| titleOfInvention | Purification process of a natamycin |
| abstract | The present invention comprises the steps of recovering the precipitate after centrifuging the culture medium containing at least 2.5 g / L, preferably 2.5 to 10 g / L natamycin per 1 L culture medium; Extracting by adding 0.5-1.2 L of methanol to 1 g of natamycin contained in the precipitate and suspending it; Increasing the solubility of natamycin by adding hydrochloric acid to the suspension containing natamycin to lower the pH to 2.5 to 3.5; Centrifuging the suspension to collect only the supernatant; Adding NaOH to the supernatant to raise the pH to about 7.0 to 8.0 and stirring for 10 to 20 minutes, followed by centrifugation to remove the precipitates; Concentrating the supernatant from which the precipitate was removed to 1/5 to 1/6 of the original volume by using a vacuum pressure concentrating device; Binding the concentrate to silica gel 60 using 15-30 g of silica gel 60 per 100 mg natamycin; Eluting impurities using distilled water and 30% (v / v) methanol sequentially in the natamycin extract column bound to silica gel; Eluting natamycin bound to silica gel 60 with 60-80% (v / v) methanol using 300 mL for 100 mg of natamycin in the amount of 60-80% (v / v) methanol used; It relates to a method for purifying natamycin, characterized in that the eluate containing natamycin is obtained by obtaining a natamycin crystal using a vacuum decompression device. |
| priorityDate | 2002-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.