http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100409092-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-555 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-77 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
| filingDate | 2000-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2003-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2003-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-100409092-B1 |
| titleOfInvention | A process for producing a protein |
| abstract | The present invention relates to a method for producing a protein, wherein a protein refolded in a single step from a cell lysate including an inclusion body and a denaturant can be obtained to prepare an active protein in a short time, simply and economically. In order to provide a method for producing a protein, (A) the process of crushing a host cell containing a protein produced in the form of an inclusion body, and adding a denaturant to the crushed host cell to unfold the aggregated protein, ( B) adsorb the released protein to the solid matrix by introducing a cell lysate containing the denaturant and the released protein into the upper direction of a column in which a solid matrix having a higher density than the cell debris is suspended in an equilibrium state in the liquid. (C) adsorbing the adsorbed protein by introducing a buffer solution containing a denaturant into the column in an upward direction; Removing the cell lysate except from the column; (d) refolding the released protein by gradually reducing the concentration of the denaturant by supplying a buffer solution containing no denaturant to the column in an upward direction; and E) providing a method for producing a protein comprising the step of obtaining the refolded protein from the column. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009005201-A1 |
| priorityDate | 2000-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 134.